Paul Hanagan's biggest success came when steering Taghrooda to triumph in the 2014 Oaks before the pair went onto King George VI And Queen Elizabeth Stakes glory later that year.
A fall back in February 2020 resulted in three fractured vertebrae and a lengthy spell out of the saddle prior to making his comeback in August.
He said: "As you can imagine it's quite emotional. It's difficult, I think any professional sportsperson will tell you, especially doing it as long as I've been doing it for.
"There's a few things involved in making my decision, I had a pretty bad fall about two years ago and I've never quite been the same after it, I fractured my back in three places.
"It's not so much painful riding, but it's getting to the level of fitness you need to be at to be a professional jockey and I don't think I was getting to that standard."
Hanagan will have his final ride onboard the Richard Fahey-trained Wootton'Sun in the opening Sky Bet Handicap at York.